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WES vs WGS: Which is More Suitable?

Key differences, advantages and disadvantages between Whole Exome and Whole Genome Sequencing

What Is the Difference Between WES and WGS?

WES (Whole Exome Sequencing) and WGS (Whole Genome Sequencing) are two advanced NGS (Next-Generation Sequencing) methods used in the diagnosis of genetic diseases. Both read DNA, but they differ significantly in scope, cost, and interpretation.

Feature WES (Whole Exome) WGS (Whole Genome)
Target RegionProtein kodlayan bölgeler (~%1-2)Tüm genom (~%100)
Base Pairs~40 milyon~3 milyar
Disease Variant Coverage%85%97+
Duration4-6 hafta6-10 hafta
Maliyet✓ Lower↗ Yüksek
Yorumlama✓ Kolay↗ Higher
Veri Depolama~1 GB~100 GB
Regulatory RegionsHayır✓ Evet
First choice (most cases)✓ EvetSeçimlik

Which Test to Choose When?

When WES Is Preferred

  • Undiagnosed patients with suspected rare disease
  • Developmental delay and intellectual disability
  • Multipl konjenital anomaliler
  • ACMG secondary findings screening
  • Situations requiring fast and economical solution

When WGS May Be Considered

  • WES negative but clinical suspicion persists
  • Suspected regulatory variants in non-coding regions
  • Homozigotite haritalama gibi ileri analiz gereksinimi
  • Need for comprehensive structural variant (SV) detection

Recommendation: Çoğu klinik durumda WES ilk tercih edilen testtir. WES negatif çıktığında ve klinik bulgular genetik bir nedeni güçlü şekilde destekliyorsa, WGS düşünülebilir. Omega Genetik'te her iki test için de danışmanlık hizmeti mevcuttur.

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Our expert genetic counselors can help you determine the most suitable test.

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